composition of bee bread

What is the bee bread composition?

Bee Bread composition refers to: moisture, protein, ashes, lipids, carbohydrates, sugars, polyphenols, amino-acids, fatty acids, minerals and vitamins. This is what bee bread is made of. It’s a superfood for bees and humans alike.

Bee bread, also found written in one word, beebread, is a less known product of the hive, equally nutritious and healthy.

Honey bees take their vital carbohydrates from nectar and pollen. The rest of of their necessary nutrients like lipids, proteins, minerals, vitamins cannot be found in nectar and will be taken almost exclusively from pollen.

How is bee bread done?

Honey bees collect pollen, mix it with their saliva and honey and then pack this mixture in the cells in the honeycomb. After 2 weeks the bee bread is made. It needs a temperature of 35 to 36 °C, moisture and the activity of different enzymes from the bees’ saliva and some microorganisms including bacteria and yeasts.

Beekeepers should always let bee bread and honey for the honey bees during winter and early spring. Young bees and worker bees use bee bread to produce royal jelly to feed larvae with it.

What is the composition of bee bread?

Bee bread is never the same, because it’s not a chemical drug prepared in a lab, following standard lines. It depends on the geographical and botanical origin of the pollen, climatic conditions, soil type, microbiota present in the cell, and also on beekeeper’s devotion and storage treatments (for commercial purpose, of course). There will always be considerable variations in the centesimal composition of beedread.

However, the are some constituents that will always be present.
– water, which represents 30% of the fresh weight;
– carbohydrates, raging from 13 to 72% of dry weight. Among them, different sugars can be found: fructose, glucose, sucrose, trehalose, and maltose, but also fructofuranose α and β, α glucopyranose, mannitol, βD-glucopyranose, melezitose, and raffinose.

The carbohydrate content of carbohydrates is increased in this product as a result of the addition of nectar and honey with the fresh pollen.
The action of α- and β-amylase and αglucosidase results in the breakdown of polysaccharides into simple sugars. Glucose and fructose are required by bacteria to ferment bee pollen into bee bread that is digested into ethanol, lactic acid, and carbon dioxide by lactic acid bacteria.
After mixing with the enzymes from the bees’ saliva, glucose and fructose can also turn into mannitol. All of these processes and probably others are responsible for the final sugar content.

Fresh Organic Bee Bread, Naturally Fermented Pollen, 460g., available on Amazon.

proteins represent between 17 and 30% of dry weight according to the reviewed literature. According to Mohammad et al., protein use is higher in bee bread than in bee pollen. However, other scientists such as Zuluaga and co-workers, say that the biochemical process induced by bees is aimed only at degrading the outer layer of the pollen grain, without causing any damage to the inner content; therefore both bee bread and bee pollen have similar protein content.

Whatever the case, the amino acidic profile is higher in bee bread, due to degradation of pollen protein which leads to more peptide and amino-acids. The microbial presence might be responsible for both the increase of amino acids through proteolysis and the decrease of amino acids, by its use as an energy source.

– lipids. The lipid content in bee bread may vary from 1.1 to 11.55% of dry weight. Scientific literature has described up to 37 fatty acids present in bee bread. Unsaturated fatty acids (UFAs) predominate over saturated fatty acids (SFAs), with up to 77.32% of total fatty acids with respect to 28.68%, respectively.

Among UFAs, there is a good correlation between monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs), with a maximum of 72.15% and a 64.70%, respectively. The
presence of some essential polyunsaturated fatty acids in bee bread is very important especially for human health. As for the honey bees, some fatty acids, like oleic and palmitic acids, are important for their nutrition.

Other acids, like myristic, linoleic, and linolenic acids are relevant because they can inhibit the growth of spore-forming bacteria.

– organic acids are also present in bee bread. There are gluconic, formic, acetic, propionic, and butyric acids and even probiotics, including lactic acid.

Essential amino acids present in bee bread: arginine, histidine, isoleucine, leucine, methionine, phenylalanine, threonine, tryptophan, valine.

Non-essential amino acids present in bee bread: alanine, aspartic acid, glutamic acid, glycine, proline, serine, tyrosine, asparagine, glutamine.

ashes represent between 1.93 and 3.42% of dry weight. An important fraction of ashes is formed by
minerals. The most abundant mineral reported for bee bread is potassium, followed by phosphorus, calcium, and magnesium. (The studies used accurate technology to analyze minerals, i.e. inductively coupled plasma mass spectrometry.) Others are also present: zinc, iron, manganese, copper, sodium and selenium.

The presence of minerals in bee pollen is the soil and the pollen gathered from flowers. In bee bread the nectar of the flowers is also added, and this is a relevant source of minerals.

– vitamins. While we have plenty of data on the presence of vitamins in bee pollen, even found in rich numbers, the available literature on bee bread has scarce info on their presence in bee bread. There are no mention on its content in various fat- and water-soluble vitamins; after an exhaustive search for vitamin values, only levels of tocopherol and vitamin C have been documented.

– phenols. Because bee pollen is one of its main components, the presence of phenolic compounds is highly expected in bee bread.

total phenolic content (mg of GAE/g) ranges from 2.53 to 13.75;
total flavonoid content (mg of QE/g) from 1.94 to 4.51;

Rutin is the main component observed, and quercetin is also present in an important concentration.

Due to bacterial action, bee bread can have a different compound proportion. Thus, Bayran et al. have observed some compounds to be higher in bee pollen, such as caffeic acid, rutin, ethyl gallate, trans-ferulic acid, and myricetin, while and in other cases higher in bee bread: protocatechuic acid, p-coumaric acid, quercetin, 2,5-dihydroxybenzoic acid, kaempferol, gallic acid, chlorogenic acid, salicylic acid, luteolin, and isorhamnetin.

463g / 1 pound Bees Bread (Perga, Bienenbrot, Ambrosia) 2022 fresh,
available on Amazon

Antioxidant power of bee bread

The presence of phenolic compounds and other ingredients are what give bee bread its antioxidant capacity. It has been described that bee bread has a similar or higher antioxidant capacity than honey
or propolis.

• TEAC (μmol of Trolox/g) from 46.13 to 76.38 (Trolox equivalent antioxidant capacity)
• FRAP (μmol of Trolox/g) from 35.03 to 70.17 (ferric-ion-reducing antioxidant power)

It has been described that bee bread extracts are more effective against Gram-positive bacteria compared to Gram negative bacteria. (Pełka, K. et all.);

Antitumoral activity has also been assayed in vitro in some studies. (Markiewicz-Żukowska, R et all.)

Bee bread has also anti-inflammatory and immunomodulatory effects, antihypertensive activity, hypolipidemic effect, and hepatoprotective actions. (Khalifa, S. A. M et all.)

What are the health benefits of bee bread?

What is the difference between beebread, aka bee bread, and bee pollen?



– Bee Products: An Emblematic Example of Underutilized Sources of Bioactive Compounds by Francesca Giampieri, Jose Luis Quiles, Danila Cianciosi, Tamara Yuliett Forbes-Hernández, Francisco Josè Orantes-Bermejo, José Miguel Alvarez-Suarez, and Maurizio Battino, Journal of Agricultural and Food Chemistry Article ASAP
DOI: 10.1021/acs.jafc.1c05822

– Pełka, K.; Otłowska, O.; Worobo, R. W.; Szweda, P. Bee Bread Exhibits Higher Antimicrobial Potential Compared to Bee Pollen. Antibiotics 2021, 10, 125;

– Markiewicz-Żukowska, R.; Naliwajko, S. K.; Bartosiuk, E.; Moskwa, J.; Isidorov, V.; Soroczynska, J.; Borawska, M. H. Chemical ́ Composition and Antioxidant Activity of Beebread, and Its Influence
on the Glioblastoma Cell Line (U87MG). J. Apic. Sci. 2013, 57, 147−157.

– Khalifa, S. A. M.; Elashal, M.; Kieliszek, M.; Ghazala, N. E.; Farag, M. A.; Saeed, A.; Xiao, J.; Zou, X.; Khatib, A.; Göransson, U.; El-Seedi, H. R. Recent Insights into Chemical and Pharmacological
Studies of Bee Bread. Trends Food Sci. Technol. 2020, 97, 300−316.

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